Nitroreductase Activites in Giardia lamblia: ORF 17150 Encodes a Quinone Reductase with Nitroreductase Activity

Müller, Joachim; Heller, Manfred; Uldry, Anne-Christine; Braga, Sophie; Müller, Norbert (2021). Nitroreductase Activites in Giardia lamblia: ORF 17150 Encodes a Quinone Reductase with Nitroreductase Activity. Pathogens, 10(2), p. 129. MDPI AG 10.3390/pathogens10020129

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The intestinal diplomonadid Giardia lamblia is a causative agent of persistent diarrhea. Current treatments are based on nitro drugs, especially metronidazole. Nitro compounds are activated by reduction, yielding toxic intermediates. The enzymatic systems responsible for this activation are not completely understood. By fractionating cell free crude extracts by size exclusion chromatography followed by mass spectrometry, enzymes with nitroreductase (NR) activities are identified. The protein encoded by ORF 17150 found in two pools with NR activities is overexpressed and characterized. In pools of fractions with main NR activities, previously-known NRs are identified, as well as a previously uncharacterized protein encoded by ORF 17150. Recombinant protein 17150 is a flavoprotein with NADPH-dependent quinone reductase and NR activities. Besides a set of previously identified NRs, we have identified a novel enzyme with NR activity.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie

UniBE Contributor:

Müller, Joachim; Heller, Manfred; Uldry, Anne-Christine; Braga, Sophie Marie-Pierre and Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2076-0817

Publisher:

MDPI AG

Funders:

Organisations 31003 not found.

Language:

English

Submitter:

Norbert Müller

Date Deposited:

12 Feb 2021 16:54

Last Modified:

12 Feb 2021 16:54

Publisher DOI:

10.3390/pathogens10020129

Uncontrolled Keywords:

anaerobic metabolism; drug susceptibility; functional assays; mass spectrometry; protein chromatography

BORIS DOI:

10.48350/151904

URI:

https://boris.unibe.ch/id/eprint/151904

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