Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier

Bittner, Aniela; Gosselet, Fabien; Sevin, Emmanuel; Dehouck, Lucie; Ducray, Angélique D.; Gaschen, Véronique; Stoffel, Michael H.; Hansang, Cho; Mevissen, Meike (2021). Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier. International journal of molecular sciences, 22(4) Molecular Diversity Preservation International MDPI 10.3390/ijms22041657

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Nanoparticle (NP)-assisted procedures including laser tissue soldering (LTS) offer advantages compared to conventional microsuturing, especially in the brain. In this study, effects of polymer-coated silica NPs used in LTS were investigated in human brain endothelial cells (ECs) and blood-brain barrier models. In the co-culture setting with ECs and pericytes, only the cell type directly exposed to NPs displayed a time-dependent internalization. No transfer of NPs between the two cell types was observed. Cell viability was decreased relatively to NP exposure duration and concentration. Protein expression of the nuclear factor k-light-chain-enhancer of activated B cells and various endothelial adhesion molecules indicated no initiation of inflammation or activation of ECs after NP exposure. Differentiation of CD34+ ECs into brain-like ECs co-cultured with pericytes, blood-brain barrier (BBB) characteristics were obtained. The established endothelial layer reduced the passage of integrity tracer molecules. NP exposure did not result in alterations of junctional proteins, BBB formation or its integrity. In a 3-dimensional setup with an endothelial tube formation and tight junctions, barrier formation was not disrupted by the NPs and NPs do not seem to cross the blood-brain barrier. Our findings suggest that these polymer-coated silica NPs do not damage the BBB.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy

UniBE Contributor:

Bittner, Aniela; Ducray, Angélique; Gaschen, Véronique; Stoffel, Michael Hubert and Mevissen, Meike

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics, prescription drugs

ISSN:

1661-6596

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Vanessa Alice Blum

Date Deposited:

12 Feb 2021 17:21

Last Modified:

12 Feb 2021 17:21

Publisher DOI:

10.3390/ijms22041657

PubMed ID:

33562136

Uncontrolled Keywords:

co-culture; 3D model; permeability; transendothelial electrical resistance

BORIS DOI:

10.48350/152019

URI:

https://boris.unibe.ch/id/eprint/152019

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