Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane

Zamprogno, Pauline; Wüthrich, Simon; Achenbach, Sven; Thoma, Giuditta; Stucki, Janick D.; Hobi, Nina; Schneider-Daum, Nicole; Lehr, klaus-michael; Huwer, Hanno; Geiser, Thomas; Schmid, Ralph A.; Guenat, Olivier T. (2021). Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane. Communications biology, 4(1), p. 168. Springer Nature 10.1038/s42003-021-01695-0

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The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Organs-on Chip Technologies

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Zamprogno, Pauline Gil Véronique; Wüthrich, Simon; Achenbach, Sven; Thoma, Giuditta; Stucki, Janick; Hobi, Nina; Geiser, Thomas; Schmid, Ralph and Guenat, Olivier Thierry

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 620 Engineering

ISSN:

2399-3642

Publisher:

Springer Nature

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

16 Feb 2021 10:33

Last Modified:

13 Mar 2021 12:51

Publisher DOI:

10.1038/s42003-021-01695-0

PubMed ID:

33547387

BORIS DOI:

10.48350/152214

URI:

https://boris.unibe.ch/id/eprint/152214

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