Bioanalysis of drugs and their metabolites by chiral electromigration techniques (2010-2020).

Caslavsky, Jitka; Thormann, Wolfgang (2021). Bioanalysis of drugs and their metabolites by chiral electromigration techniques (2010-2020). Electrophoresis, 42(17-18), pp. 1744-1760. Wiley-VCH 10.1002/elps.202000383

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The further development and application of capillary electromigration techniques for the enantioselective determination of drugs and their metabolites in body fluids, tissues and in vitro preparations during the 2010 to 2020 time period continued to proof their usefulness and attractiveness in bioanalysis. This review discusses the principles and important aspects of capillary electrophoresis based chiral drug bioassays, provides a survey of the assays reported during the past 10 years and presents an overview of the key achievements encountered in that time period. For systems with charged chiral selectors, special attention is paid on assays that feature field-amplified sample stacking to enable the determination of ppb levels of analytes and optimized on-line incubation procedures for the rapid assessment of a metabolic pathway. Applications discussed encompass the pharmacokinetics of drug enantiomers in vivo and in vitro, the impact of inhibitors on metabolic steps, the elucidation of the stereoselectivity of drug metabolism in vivo and in vitro, and drug enantiomers in toxicological, forensic and doping analysis. This article is protected by copyright. All rights reserved.

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