Cooperative and Noncooperative Assembly of Oligopyrenotides Resolved by Atomic Force Microscopy

Rudnev, Alexander; Malinovskii, Vladimir L.; Nussbaumer, Alina Laura; Mishchenko, Artem; Häner, Robert; Wandlowski, Thomas (2012). Cooperative and Noncooperative Assembly of Oligopyrenotides Resolved by Atomic Force Microscopy. Macromolecules, 45(15), pp. 5986-5992. Washington, D.C.: American Chemical Society 10.1021/ma3007619

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The supramolecular assembly of amphiphilic oligopyrenotide building blocks (covalently linked heptapyrene, Py7) is studied by atomic force microscopy (AFM) in combination with optical spectroscopy. The assembly process is triggered in a controlled manner by increasing the ionic strength of the aqueous oligomer solution. Cooperative noncovalent interactions between individual oligomeric units lead to the formation of DNA-like supramolecular polymers. We also show that the terminal attachment of a single cytidine nucleotide to the heptapyrenotide (Py7-C) changes the association process from a cooperative (nucleation−elongation) to a noncooperative (isodesmic) regime, suggesting a structure misfit between the cytidine and the pyrene units. We also demonstrate that AFM enables the identification and characterization of minute concentrations of the supramolecular products, which was not accessible by conventional optical spectroscopy.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Rudnev, Alexander; Nussbaumer, Alina Laura; Mishchenko, Artem; Häner, Robert and Wandlowski, Thomas

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0024-9297

Publisher:

American Chemical Society

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:38

Last Modified:

31 Dec 2014 09:16

Publisher DOI:

10.1021/ma3007619

Web of Science ID:

000307479100018

BORIS DOI:

10.7892/boris.15278

URI:

https://boris.unibe.ch/id/eprint/15278 (FactScience: 222586)

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