Component‐resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity

Novotny, Ella N.; White, Samuel J.; Wilson, A. Douglas; Stefánsdóttir, Sara B.; Tijhaar, Edwin; Jonsdóttir, Sigridur; Frey, Rebekka; Reiche, Dania; Rose, Horst; Rhyner, Claudio; Schüpbach, Gertraud; Torsteinsdóttir, Sigurbjörg; Alcocer, Marcos; Marti, Eliane (2021). Component‐resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity. Allergy, 76(4), pp. 1147-1157. Wiley-Blackwell 10.1111/all.14556

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Background: Allergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray.

Methods: IgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis.

Results: Seropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91).

Conclusion: This microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Novotny, Ella Nina; Jonsdottir, Sigridur; Schüpbach, Gertraud Irene and Marti, Eliane Isabelle

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture
500 Science > 590 Animals (Zoology)

ISSN:

0105-4538

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eliane Isabelle Marti Schalch

Date Deposited:

23 Mar 2021 15:40

Last Modified:

13 Apr 2021 01:34

Publisher DOI:

10.1111/all.14556

PubMed ID:

32780483

BORIS DOI:

10.48350/153057

URI:

https://boris.unibe.ch/id/eprint/153057

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