Carbon Source-Dependent Changes of the Structure of Streptococcus pneumoniae Capsular Polysaccharide with Serotype 6F

Werren, Joel P.; Troxler, Lukas J.; Oyewole, Oluwaseun Rume-Abiola; Ramette, Alban; Brugger, Silvio D.; Bruggmann, Rémy; van der Linden, Mark; Nahm, Moon H.; Gjuroski, Ilche; Casanova, Carlo; Furrer, Julien; Hilty, Markus (2021). Carbon Source-Dependent Changes of the Structure of Streptococcus pneumoniae Capsular Polysaccharide with Serotype 6F. International journal of molecular sciences, 22(9) MDPI 10.3390/ijms22094580

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The structure of the exopolysaccharide capsule of Streptococcus pneumoniae is defined by the genetic arrangement of the capsule operon allowing the unequivocal identification of the pneumococcal serotype. Here, we investigated the environment-dependent composition of the polysaccharide structure of S. pneumoniae serotype 6F. When grown in a chemically defined medium (CDM) with glucose versus galactose, the exopolysaccharide capsule of the serotype 6F strains reveals a ratio of 1/0.6 or 1/0.3 for galactose/glucose in the capsule by 1H-NMR analyses, respectively. Increased production of the capsule precursor UDP-glucose has been identified by 31P-NMR in CDM with glucose. Flow cytometric experiments using monoclonal antibodies showed decreased labelling of Hyp6AG4 (specific for serotype 6A) antibodies when 6F is grown in glucose as compared to galactose, which mirrors the 1H-NMR results. Whole-genome sequencing analyses of serotype 6F isolates suggested that the isolates evolved during two different events from serotype 6A during the time when the 13-valent pneumococcal conjugate vaccine (PCV-13) was introduced. In conclusion, this study shows differences in the capsular structure of serotype 6F strains using glucose as compared to galactose as the carbon source. Therefore, 6F strains may show slightly different polysaccharide composition while colonizing the human nasopharynx (galactose rich) as compared to invasive locations such as the blood (glucose rich).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Clinical Microbiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > General Bacteriology
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Werren, Joel Pascal; Troxler, Lukas; Oyewole, Oluwaseun Rume-Abiola; Ramette, Alban Nicolas; Bruggmann, Rémy; Gjuroski, Ilche; Casanova, Carlo; Furrer, Julien and Hilty, Markus

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
500 Science > 540 Chemistry

ISSN:

1422-0067

Publisher:

MDPI

Language:

English

Submitter:

Julien Henri Lucien Furrer

Date Deposited:

06 May 2021 11:52

Last Modified:

06 May 2021 11:52

Publisher DOI:

10.3390/ijms22094580

PubMed ID:

33925509

BORIS DOI:

10.48350/156136

URI:

https://boris.unibe.ch/id/eprint/156136

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