EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs

Gantenbein, Benjamin; May, Rahel D.; Bermudez-Lekerika, Paola; Oswald, Katharina A. C.; Benneker, Lorin M.; Albers, Christoph E. (2021). EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs. Applied Sciences, 11(9), p. 4072. MDPI 10.3390/app11094072

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Diffuse idiopathic skeletal hyperostosis (DISH) is characterised by ectopic ossificationalong the anterior spine and the outer intervertebral discs (IVD). However, the centre of the IVD,i.e., the nucleus pulposus, always remains unaffected, which could be due to the inhibition of thebone morphogenetic protein (BMP) pathway. In this study, we investigated the transcriptome forthe BMP pathway of DISH-IVD cells versus disc cells of traumatic or degenerative discs. The disccells originated from nucleus pulposus (NP), annulus fibrosus (AF) and from cartilaginous endplate(CEP) tissue. Here, ninety genes of the transforming growth factorβ-BMP signalling pathway werescreened by qPCR. Furthermore, the protein expression of genes of interest was further investigatedby immune-staining and semi-quantitative microscopy. IVDs of three DISH patients were testedagainst three control patients (same disc level and similar age). Early Growth Response 2 (EGR2)and Interleukin 6 (IL6) were both significantly up-regulated in DISH-IVD cells compared to controls(12.8±7.6-fold and 54.0±46.5-fold, respectively, means±SEM). Furthermore, Insulin-like GrowthFactor 1 (IGF1) tended to be up-regulated in DISH-IVD donors, i.e., 174.13±120.6-fold. IGF1 wasalready known as a serum marker for DISH and other rheumatoid diseases and is confirmed here toplay a possible key role in DISH-IVD.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB [discontinued]
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Orthopädische Chirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Orthopädische Chirurgie

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Live Cell Imaging (LCI)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Gantenbein, Benjamin; May, Rahel Deborah; Bermudez, Paola; Oswald, Katharina Anna Christine; Benneker, Lorin Michael and Albers, Christoph

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2076-3417

Publisher:

MDPI

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

11 May 2021 08:40

Last Modified:

16 May 2021 03:04

Publisher DOI:

10.3390/app11094072

BORIS DOI:

10.48350/156197

URI:

https://boris.unibe.ch/id/eprint/156197

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