Pasch, Andreas; Farese, Stefan; Gräber, Steffen; Wald, Johanna; Richtering, Walter; Floege, Jürgen; Jahnen-Dechent, Willi (2012). Nanoparticle-based test measures overall propensity for calcification in serum. Journal of the American Society of Nephrology, 23(10), pp. 1744-52. Hagerstown, Md.: Lippincott Williams & Wilkins 10.1681/ASN.2012030240
Full text not available from this repository.Vascular and soft tissue calcification contributes to cardiovascular morbidity and mortality in both the general population and CKD. Because calcium and phosphate serum concentrations are near supersaturation, the balance of inhibitors and promoters critically influences the development of calcification. An assay that measures the overall propensity for calcification to occur in serum may have clinical use. Here, we describe a nanoparticle-based assay that detects, in the presence of artificially elevated calcium and phosphate concentrations, the spontaneous transformation of spherical colloidal primary calciprotein particles (CPPs) to elongate crystalline secondary CPPs. We used characteristics of this transition to describe the intrinsic capacity of serum to inhibit the precipitation of calcium and phosphate. Using this assay, we found that both the sera of mice deficient in fetuin-A, a serum protein that inhibits calcification, and the sera of patients on hemodialysis have reduced intrinsic properties to inhibit calcification. In summary, we developed a nanoparticle-based test that measures the overall propensity for calcification in serum. The clinical use of the test requires evaluation in a prospective study.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension |
UniBE Contributor: |
Pasch, Andreas |
ISSN: |
1046-6673 |
Publisher: |
Lippincott Williams & Wilkins |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:39 |
Last Modified: |
05 Dec 2022 14:12 |
Publisher DOI: |
10.1681/ASN.2012030240 |
PubMed ID: |
22956818 |
Web of Science ID: |
000309736000020 |
URI: |
https://boris.unibe.ch/id/eprint/15625 (FactScience: 223029) |
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