A filter-assisted culture method for isolation of Treponema spp. from bovine digital dermatitis and their identification by MALDI-TOF MS.

Brodard, Isabelle; Alsaaod, Maher; Gurtner, Corinne; Jores, Joerg; Steiner, Adrian; Kuhnert, Peter (2021). A filter-assisted culture method for isolation of Treponema spp. from bovine digital dermatitis and their identification by MALDI-TOF MS. Journal of veterinary diagnostic investigation, 33(4), pp. 801-805. Sage 10.1177/10406387211008511

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Digital dermatitis (DD) is a major infectious foot disease of cattle worldwide. Some DD stages are associated with lameness, and the disease has significant economic and animal welfare consequences. The pathogenesis of the disease is not yet fully understood, but Treponema spp. have been associated consistently with clinical cases. Isolation of these fastidious bacteria is difficult and cumbersome. We describe an improved method enabling the culturing of the 3 Treponema spp. (T. pedis, T. phagedenis, and T. medium) from bovine foot specimens derived from DD lesions, using a combination of membrane filtering and subsequent growth on selective agar media. The entire procedure from sampling to verification of individual Treponema spp. takes up to 24 d. In addition, we established a MALDI-TOF MS-based identification method to be applied for confirmation of the different Treponema spp. This scheme provides an unambiguous, simple, and straightforward identification procedure for DD-associated Treponema spp.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Brodard, Isabelle; Alsaaod, Maher; Gurtner, Corinne; Jores, Jörg; Steiner, Adrian and Kuhnert, Peter

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1943-4936

Publisher:

Sage

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

26 May 2021 08:52

Last Modified:

25 Jun 2021 01:33

Publisher DOI:

10.1177/10406387211008511

PubMed ID:

33834899

Uncontrolled Keywords:

MALDI-TOF MS Treponema spp cattle culture digital dermatitis non-healing lesion

BORIS DOI:

10.48350/156509

URI:

https://boris.unibe.ch/id/eprint/156509

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