A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics

Reuther, Julia; Schneider, Lukas; Iacovache, Ioan; Pircher, Andreas; Gharib, Walid H.; Zuber, Benoît; Polacek, Norbert (2021). A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics. RNA biology, pp. 1-16. Taylor and Francis 10.1080/15476286.2021.1935573

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Ribosome-associated non-coding RNAs (rancRNAs) have been recognized as an emerging class of regulatory molecules capable of fine-tuning translation in all domains of life. RancRNAs are ideally suited for allowing a swift response to changing environments and are therefore considered pivotal during the first wave of stress adaptation. Previously, we identified an mRNA-derived 18 nucleotides long rancRNA (rancRNA_18) in Saccharomyces cerevisiae that rapidly downregulates protein synthesis during hyperosmotic stress. However, the molecular mechanism of action remained enigmatic. Here, we combine biochemical, genetic, transcriptome-wide and structural evidence, thus revealing rancRNA_18 as global translation inhibitor by targeting the E-site region of the large ribosomal subunit. Ribosomes carrying rancRNA_18 possess decreased affinity for A-site tRNA and impaired structural dynamics. Cumulatively, these discoveries reveal the mode of action of a rancRNA involved in modulating protein biosynthesis at a thus far unequalled precision.

Item Type:

Journal Article (Original Article)

Division/Institute:

09 Interdisciplinary Units > Microscopy Imaging Center (MIC)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
08 Faculty of Science > Department of Biology > Bioinformatics

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Reuther, Julia; Schneider, Lukas; Pircher, Andreas; Zuber, Benoît and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
600 Technology > 610 Medicine & health

ISSN:

1555-8584

Publisher:

Taylor and Francis

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

24 Jun 2021 10:57

Last Modified:

29 Jun 2021 16:01

Publisher DOI:

10.1080/15476286.2021.1935573

BORIS DOI:

10.48350/157017

URI:

https://boris.unibe.ch/id/eprint/157017

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