General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds

Guex, Anne Géraldine; Fortunato, Giuseppino; Hegemann, Dirk; Tevaearai, Hendrik T; Giraud, Marie-Noëlle (2013). General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds. In: Stem Cell Nanotechnology 1058 (pp. 119-131). Totowa, N.J.: Humana Press 10.1007/7651_2013_8

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As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electrospun scaffolds. We describe a method to prepare and fix the scaffolds in culture wells and discuss a convenient method for cell seeding and subsequent analysis by scanning electron microscopy or immunohistology.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery

UniBE Contributor:

Guex, Anne Géraldine and Tevaearai, Hendrik

ISSN:

1064-3745

ISBN:

978-1-62703-570-5

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:39

Last Modified:

08 Jun 2016 10:33

Publisher DOI:

10.1007/7651_2013_8

PubMed ID:

23700279

URI:

https://boris.unibe.ch/id/eprint/15704 (FactScience: 223133)

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