Exploring the global spread of Klebsiella grimontii isolates possessing blaVIM-1 and mcr-9.

Campos-Madueno, Edgar I.; Moser, Aline I.; Risch, Martin; Bodmer, Thomas; Endimiani, Andrea (2021). Exploring the global spread of Klebsiella grimontii isolates possessing blaVIM-1 and mcr-9. Antimicrobial agents and chemotherapy, 65(9), e0072421. American Society for Microbiology 10.1128/AAC.00724-21

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The spread of plasmid-mediated carbapenemases within Klebsiella oxytoca is well-documented. In contrast, data concerning the closely-related species Klebsiella grimontii are scarce. In fact, despite the recent report of the first blaKPC-2-producing K. grimontii, nothing is known about its clonality and antibiotic resistance patterns. In a retrospective search in our collection, we identified 2 blaVIM-positive K. oxytoca strains. Whole-genome sequencing with both Illumina and Nanopore indicated that our strains actually belonged to K. grimontii and were of sequence type (ST) 172 and ST189. Moreover, the two strains were associated with 297kb IncHI2/HI2A-pST1 and 90.6kb IncFII(Yp) plasmids carrying blaVIM-1 together with mcr-9 and blaVIM-1, respectively. In the IncHI2/HI2A plasmid, blaVIM-1 was located in a class 1 integron (In110), while mcr-9 was associated to the qseC-qseB-like regulatory elements. Overall, this plasmid showed to be very similar to those carried by other Enterobacterales isolated from food and animal sources (e.g., Salmonella and Enterobacter spp. detected in Germany and Egypt). The IncFII(Yp) plasmid was unique and its blaVIM-1 region was associated to a rare integron (In1373). Mapping of In1373 indicated a possible origin in Austria from an Enterobacter hormaechei carrying a highly similar plasmid. Core-genome phylogenies indicated that the ST172 K. grimontii belonged to a clone of identical Swedish and Swiss strains (≤15 SNVs to each other), whereas the ST189 strain was sporadic. Surveillance of carbapenemase-producing K. oxytoca strains should be reinforced to detect and prevent the dissemination of new species belonging to the Klebsiella genus.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Clinical Microbiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > General Bacteriology

UniBE Contributor:

Campos-Madueno, Edgar Igor; Moser, Aline and Endimiani, Andrea

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0066-4804

Publisher:

American Society for Microbiology

Funders:

[4] Swiss National Science Foundation

Projects:

[1124] Whole Genome and Plasmid Sequencing for MDR Enterobacteriaceae Simultaneously Isolated from Multiple Human and Non-Human Settings: Deciphering Impact, Risks, and Dynamics for Resistance Transmission and Spread
[1501] Multidrug-Resistant Enterobacterales Colonizing Swiss Embassy Employees and Relatives Worldwide: Molecular Features, Metagenomics, and Transmission to the Householders at Return Official URL

Language:

English

Submitter:

Andrea Endimiani

Date Deposited:

06 Jul 2021 15:07

Last Modified:

19 Aug 2021 01:33

Publisher DOI:

10.1128/AAC.00724-21

PubMed ID:

34181480

BORIS DOI:

10.48350/157263

URI:

https://boris.unibe.ch/id/eprint/157263

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