Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera).

Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; Invernizzi, Ciro; Reynaldi, Francisco José; Le Conte, Yves; Beaurepaire, Alexis (2021). Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera). Microorganisms, 9(5) MDPI 10.3390/microorganisms9051087

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RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Bee Health

UniBE Contributor:

Beaurepaire, Alexis

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

2076-2607

Publisher:

MDPI

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

14 Jul 2021 09:58

Last Modified:

14 Jul 2021 10:06

Publisher DOI:

10.3390/microorganisms9051087

PubMed ID:

34070128

Uncontrolled Keywords:

evolutionary biology host–pathogen interactions pollinators population genetics viruses

BORIS DOI:

10.48350/157551

URI:

https://boris.unibe.ch/id/eprint/157551

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