Putative Novel Atypical BTV Serotype '36' Identified in Small Ruminants in Switzerland.

Ries, Christina; Vögtlin, Andrea; Hüssy, Daniela; Jandt, Tabea; Gobet, Hansjörg; Hilbe, Monika; Burgener, Carole; Schweizer, Luzia; Häfliger-Speiser, Stephanie; Beer, Martin; Hoffmann, Bernd (2021). Putative Novel Atypical BTV Serotype '36' Identified in Small Ruminants in Switzerland. Viruses, 13(5) Molecular Diversity Preservation International MDPI 10.3390/v13050721

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We identified a putative novel atypical BTV serotype '36' in Swiss goat flocks. In the initial flock clinical signs consisting of multifocal purulent dermatitis, facial oedema and fever were observed. Following BTV detection by RT-qPCR, serotyping identified BTV-25 and also a putative novel BTV serotype in several of the affected goats. We successfully propagated the so-called "BTV-36-CH2019" strain in cell culture, developed a specific RT-qPCR targeting Segment 2, and generated the full genome by high-throughput sequencing. Furthermore, we experimentally infected goats with BTV-36-CH2019. Regularly, EDTA blood, serum and diverse swab samples were collected. Throughout the experiment, neither fever nor clinical disease was observed in any of the inoculated goats. Four goats developed BTV viremia, whereas one inoculated goat and the two contact animals remained negative. No viral RNA was detected in the swab samples collected from nose, mouth, eye, and rectum, and thus the experimental infection of goats using this novel BTV serotype delivered no indications for any clinical symptoms or vector-free virus transmission pathways. The subclinical infection of the four goats is in accordance with the reports for other atypical BTVs. However, the clinical signs of the initial goat flock did most likely not result from infection with the novel BTV-36-CH0219.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Vögtlin, Andrea and Hüssy, Daniela

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1999-4915

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

09 Aug 2021 15:50

Last Modified:

09 Aug 2021 16:06

Publisher DOI:

10.3390/v13050721

PubMed ID:

33919269

Uncontrolled Keywords:

BTV atypical BTV bluetongue virus goat novel serotype serotype 36 small ruminant

BORIS DOI:

10.48350/157743

URI:

https://boris.unibe.ch/id/eprint/157743

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