Methylome dynamic upon proteasome inhibition by the Pseudomonas syringae virulence factor Syringolin A

Bonnet, Diane M. V. J.; Grob, S; Tirot, Louis; Jullien, Pauline E. (1 April 2021). Methylome dynamic upon proteasome inhibition by the Pseudomonas syringae virulence factor Syringolin A (bioRxiv). Cold Spring Harbor Laboratory 10.1101/2021.01.19.427327

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DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. DNA methylation patterns can be modified by environmental factors such as pathogen infection, where modification of DNA methylation can be associated with plant resistance. To counter the plant defense pathways, pathogens produce effectors molecule, several of which act as proteasome inhibitors. Here we investigated the effect of proteasome inhibition by the bacterial virulence factor Syringolin A on genome-wide DNA methylation. We show that Syringolin A treatment results in an increase of DNA methylation at centromeric and pericentromeric regions of Arabidopsis chromosomes. We identify several CHH DMRs that are enriched in the proximity of transcriptional start sites. Syringolin A treatment does not result in significant changes in small RNA composition. However, significant changes in genome transcriptional activity can be observed, including a strong upregulation of resistance genes that are located on chromosomal arms. We hypothesize that DNA methylation changes could be linked to the upregulation of some atypical members of the de novo DNA methylation pathway: AGO3, AGO9 and DRM1. Our data suggests that modification of genome-wide DNA methylation resulting from an inhibition of the proteasome by bacterial effectors could be part of a epi-genomic arms race against pathogens.Competing Interest StatementThe authors have declared no competing interest.

Item Type:

Working Paper

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Epigenetics
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Bonnet, Diane Marie Valérie J.; Tirot, Louis Jean François and Jullien, Pauline Emilie

Subjects:

500 Science > 580 Plants (Botany)

Series:

bioRxiv

Publisher:

Cold Spring Harbor Laboratory

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

11 Aug 2021 09:43

Last Modified:

11 Aug 2021 09:43

Publisher DOI:

10.1101/2021.01.19.427327

Uncontrolled Keywords:

DNA methylation, Arabidopsis, Syringolin A, proteasome inhibition, bacterial defense

BORIS DOI:

10.48350/157963

URI:

https://boris.unibe.ch/id/eprint/157963

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