Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering

Benítez-Mateos, Ana I.; Bertella, Stefania; Behaghel de Bueren, Jean; Luterbacher, Jeremy S.; Paradisi, Francesca (2021). Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering. ChemSusChem, 14(15), pp. 3198-3207. Wiley-VCH 10.1002/cssc.202100926

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Lignin has emerged as an attractive alternative in the search for more eco-friendly and less costly materials for enzyme immobi- lization. In this work, the terephthalic aldehyde-stabilization of lignin is carried out during its extraction to develop a series of functionalized lignins with a range of reactive groups (epoxy, amine, aldehyde, metal chelates). This expands the immobiliza- tion to a pool of enzymes (carboxylase, dehydrogenase, trans- aminase) by different binding chemistries, affording immobiliza- tion yields of 64–100%. As a proof of concept, a ω- transaminase reversibly immobilized on polyethyleneimine- lignin is integrated in a packed-bed reactor. The stability of the immobilized biocatalyst is tested in continuous-flow deamina- tion reactions and maintains the same conversion for 100 cycles. These results outperform previous stability tests carried out with the enzyme covalently immobilized on methacrylic resins, with the advantage that the reversibility of the immobilized enzyme allows recycling and reuse of lignin beyond the enzyme inactivation. Additionally, an in-line system also based on lignin is added into the downstream process to separate the reaction products by catch-and-release. These results demonstrate a fully closed-loop sustainable flow- biocatalytic system based exclusively on lignin.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Benitez Mateos, Ana Isabel and Paradisi, Francesca

Subjects:

500 Science > 540 Chemistry

ISSN:

1864-5631

Publisher:

Wiley-VCH

Funders:

[4] Swiss National Science Foundation ; [185] University of Bern: Seal of Excellence Fund

Projects:

[UNSPECIFIED] 200021_192274
[UNSPECIFIED] SELF19-03 BIORPHAN- DRUG
[UNSPECIFIED] CRSII5_180258

Language:

English

Submitter:

Francesca Paradisi

Date Deposited:

20 Aug 2021 15:07

Last Modified:

20 Aug 2021 15:14

Publisher DOI:

10.1002/cssc.202100926

BORIS DOI:

10.48350/158313

URI:

https://boris.unibe.ch/id/eprint/158313

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