Salivary Pellicle Modification with Grape-seed Extract: In Vitro Study on the Effect on Bacterial Adhesion and Biofilm Formation

Saads Carvalho, Thiago; Muçolli, Dea; Eick, Sigrun; Baumann, Tommy (2021). Salivary Pellicle Modification with Grape-seed Extract: In Vitro Study on the Effect on Bacterial Adhesion and Biofilm Formation. Oral health & preventive dentistry, 19(1), pp. 301-309. Quintessenz Verlags-GmbH 10.3290/j.ohpd.b1453013

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Purpose: Grape-seed extract (GSE) contains polyphenols that readily adhere to proteins and modify the acquired enamel pellicle (AEP). The first step in biofilm formation is bacterial adhesion to the AEP-covered enamel. The aim of this in vitro study was to test whether AEP modification with GSE, fluoride (F-), or their combination (GSE+F-) modulates bacterial adhesion, biofilm metabolism and composition, or cariogenic demineralisation of the enamel.

Materials and methods: The study comprised 3 parts: 1) single-strain Streptococcus gordonii species, 2) a five-species biofilm model, or 3) biofilm (re-)formation using the five-species biofilm model after removal of initial biofilm with toothbrushing. Human whole-mouth stimulated saliva was used to form an AEP on human enamel specimens. The AEP was incubated in water (control), or modified with GSE, F-, or GSE+F-. Bacterial adhesion, biofilm diversity, metabolic activity, biofilm mass, and cariogenic demineralisation (surface hardness) of enamel were assessed after incubation in bacterial broths after 4 h or 22 h. Differences between groups were analysed with one-way ANOVA and post-hoc Bonferroni tests.

Results: GSE and GSE+F- statistically significantly decreased single-strain S. gordonii adhesion, but had no relevant influence when the five-species biofilm model was used. In the biofilm (re-)formation model, GSE reduced bacterial adhesion compared to GSE+F-, while F- caused less cariogenic demineralisation than was found in the control group.

Conclusion: AEP modified with GSE retards S. gordonii adhesion, but it does not influence the formation, metabolism and composition of a cariogenic multi-species biofilm.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine > Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research

UniBE Contributor:

Saads Carvalho, Thiago; Muçolli, Dea; Eick, Sigrun and Baumann, Tommy

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1602-1622

Publisher:

Quintessenz Verlags-GmbH

Language:

English

Submitter:

Thiago Saads Carvalho

Date Deposited:

31 Aug 2021 17:04

Last Modified:

05 Sep 2021 03:05

Publisher DOI:

10.3290/j.ohpd.b1453013

PubMed ID:

34057340

BORIS DOI:

10.48350/159073

URI:

https://boris.unibe.ch/id/eprint/159073

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