Minimal peptide length required to span the mitochondrial protein translocases in Trypanosoma brucei

Wenger, Christoph; Schneider, André (2021). Minimal peptide length required to span the mitochondrial protein translocases in Trypanosoma brucei. Molecular and biochemical parasitology, 244, p. 111393. Elsevier 10.1016/j.molbiopara.2021.111393

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Mitochondrial protein import depends on heterooligomeric translocases in the outer and inner membranes. Using import substrates consisting of various lengths of the N-terminal part of mitochondrial dihydrolipoamide dehydrogenase (LDH) fused to dihydrofolate reductase we present an in vivo analysis showing that in Trypanosoma brucei at least 96 aa of mature LDH are required to efficiently produce an import intermediate that spans both translocases. This is different to yeast, where around 50 aa are sufficient to achieve the same task and likely reflects the different arrangement and architecture of the trypanosomal mitochondrial translocases. Furthermore, we show that formation of the stuck import intermediate leads to a strong growth inhibition suggesting that, depending on the length of the LDH, the import channels in the translocases are quantitatively blocked.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wenger, Christoph and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

01 Oct 2021 16:21

Last Modified:

03 Oct 2021 03:01

Publisher DOI:

10.1016/j.molbiopara.2021.111393

PubMed ID:

34197864

BORIS DOI:

10.48350/159484

URI:

https://boris.unibe.ch/id/eprint/159484

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