Searching for synergy: combining systemic daptomycin treatment with localised phage therapy for the treatment of experimental pneumonia due to MRSA.

Valente, Luca G.; Federer, Lea; Iten, Manuela; Grandgirard, Denis; Leib, Stephen L.; Jakob, Stephan M.; Haenggi, Matthias; Cameron, David R.; Que, Yok-Ai; Prazak, Josef (2021). Searching for synergy: combining systemic daptomycin treatment with localised phage therapy for the treatment of experimental pneumonia due to MRSA. BMC research notes, 14(1), p. 381. Biomed Central 10.1186/s13104-021-05796-1

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OBJECTIVE

Bacteriophages (or phages) are viruses which infect and lyse bacteria. The therapeutic use of phages (phage therapy) has regained attention in the last decades as an alternative strategy to treat infections caused by antimicrobial-resistant bacteria. In clinical settings it is most likely that phages are administered adjunct to antibiotics. For successful phage therapy it is therefore crucial to investigate different phage-antibiotic combinations in vivo. This study aimed to elucidate the combinatorial effects of systemic daptomycin and nebulised bacteriophages for the treatment of experimental pneumonia due to methicillin-resistant Staphylococcus aureus (MRSA).

RESULTS

Using a rat model of ventilator-associated pneumonia caused by MRSA, the simultaneous application of intravenous daptomycin and nebulised phages was not superior to aerophage therapy alone at improving animal survival (55% vs. 50%), or reducing bacterial burdens in the lungs, or spleen. Thus, this combination does not seem to be of benefit for use in patients with MRSA pneumonia.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Forschungsgruppe Intensivmedizin

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Valente, Luca Gabriele; Iten, Manuela; Grandgirard, Denis; Leib, Stephen; Jakob, Stephan; Hänggi, Matthias; Cameron, David Robert; Que, Yok-Ai and Prazak, Josef

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1756-0500

Publisher:

Biomed Central

Funders:

[4] Swiss National Science Foundation ; [UNSPECIFIED] Novartis Foundation for Medical Research ; [UNSPECIFIED] ESICM Basic Science Award

Language:

English

Submitter:

Stephen Leib

Date Deposited:

03 Nov 2021 16:57

Last Modified:

07 Nov 2021 01:40

Publisher DOI:

10.1186/s13104-021-05796-1

PubMed ID:

34579784

Uncontrolled Keywords:

Methicillin-resistant Staphylococcus aureus Nebulized bacteriophages Phage-antibiotic interactions Ventilator-associated pneumonia

BORIS DOI:

10.48350/159986

URI:

https://boris.unibe.ch/id/eprint/159986

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