Russo, Fabrizio; Ambrosio, Luca; Peroglio, Marianna; Guo, Wei; Wangler, Sebastian; Gewiess, Jan; Grad, Sibylle; Alini, Mauro; Papalia, Rocco; Vadalà, Gianluca; Denaro, Vincenzo (2021). A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study. International journal of molecular sciences, 22(6) MDPI 10.3390/ijms22062963
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The purpose of the present pilot study was to evaluate the effect of a hydrogel composed of hyaluronic acid (HA) and platelet-rich plasma (PRP) as a carrier for human mesenchymal stem cells (hMSCs) for intervertebral disc (IVD) regeneration using a disc organ culture model. HA was mixed with batroxobin (BTX) and PRP to form a hydrogel encapsulating 1 × 106 or 2 × 106 hMSCs. Bovine IVDs were nucleotomized and filled with hMSCs suspended in ~200 μL of the PRP/HA/BTX hydrogel. IVDs collected at day 0 and nucleotomized IVDs with no hMSCs and/or hydrogel alone were used as controls. hMSCs encapsulated in the hydrogel were also cultured in well plates to evaluate the effect of the IVD environment on hMSCs. After 1 week, tissue structure, scaffold integration, hMSC viability and gene expression of matrix and nucleus pulposus (NP) cell markers were assessed. Histological analysis showed a better preservation of the viability of the IVD tissue adjacent to the gel in the presence of hMSCs (~70%) compared to the hydrogel without hMSCs. Furthermore, disc morphology was maintained, and the hydrogel showed signs of integration with the surrounding tissues. At the gene expression level, the hydrogel loaded with hMSCs preserved the normal metabolism of the tissue. The IVD environment promoted hMSC differentiation towards a NP cell phenotype by increasing cytokeratin-19 (KRT19) gene expression. This study demonstrated that the hydrogel composed of HA/PRP/BTX represents a valid carrier for hMSCs being able to maintain a good cell viability while stimulating cell activity and NP marker expression.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery |
UniBE Contributor: |
Wangler, Sebastian, Gewiess, Jan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Kathrin Aeschlimann |
Date Deposited: |
10 Nov 2021 17:00 |
Last Modified: |
05 Dec 2022 15:53 |
Publisher DOI: |
10.3390/ijms22062963 |
PubMed ID: |
33803999 |
Uncontrolled Keywords: |
ex vivo hyaluronic acid intervertebral disc mesenchymal stem cells organ culture platelet-rich plasma |
BORIS DOI: |
10.48350/160110 |
URI: |
https://boris.unibe.ch/id/eprint/160110 |
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