Duration of carriage of multidrug-resistant bacteria in dogs and cats in veterinary care and co-carriage with their owners.

Dazio, Valentina; Nigg, Aurélien; Schmidt, Janne S; Brilhante, Michael; Campos-Madueno, Edgar I.; Mauri, Nico; Kuster, Stefan P; Gobeli Brawand, Stefanie; Willi, Barbara; Endimiani, Andrea; Perreten, Vincent; Schuller, Simone (2021). Duration of carriage of multidrug-resistant bacteria in dogs and cats in veterinary care and co-carriage with their owners. One health, 13(100322), p. 100322. Elsevier 10.1016/j.onehlt.2021.100322

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Background

The emergence and spread of multidrug-resistant organisms (MDROs) represent a threat to human and animal health.

Objectives

To assess duration of carriage of MDROs in dogs and cats presented to veterinary clinics/hospitals in Switzerland. To estimate prevalence, duration of and risk factors for MDRO carriage in their owners and the occurrence of co-carriage in owner-pet pairs.

Methods

Prospective, longitudinal, observational study. Nasal swabs and fecal samples were collected from 50 owners of dogs and cats presented to 3 large veterinary hospitals, 1 medium-sized clinic and 1 practice. If pet or owner tested positive for a MDRO, follow-up samples were collected for up to 8 months. Methicillin-resistant (MR) Staphylococcus aureus, MR S. pseudintermedius, MR coagulase-negative staphylococci (MRCoNS), MR Macrococcus spp., cephalosporinase- and carbapenemase-producing (CP) Enterobacterales were isolated and further characterized by MALDI-TOF MS, microdilution, β-lactam resistance gene detection, REP/ERIC-PCR, multilocus sequence typing or whole-genome sequencing. Risk factors for MDRO carriage in owners were explored based on questionnaire-derived data.

Results

Five out of 50 owners carried 3rd generation cephalosporin-resistant Enterobacterales (3GC-R-Ent.), and 5/50 MRCoNS. In 3 dogs and 4 cats carriage of 3GC-R-Ent. persisted for up to 136 days after discharge (median 99 days, IQR 83 days, range 36-136 days), in two cats isolates were carbapenem-resistant. Owner-pet co-carriage was not observed. No specific risk factors for MDRO carriage in owners were identified.

Conclusions

After discharge from veterinary care, dogs and cats may carry 3GC-R-Ent. for prolonged time periods. Carriage of MDROs was common in owners, but pet-owner co-carriage of the same MDRO was not observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Anaesthesiology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Dazio, Valentina Elisa; Quinta Brilhante, Michael; Campos-Madueno, Edgar Igor; Gobeli, Stefanie; Endimiani, Andrea; Perreten, Vincent and Schuller, Simone

Subjects:

600 Technology > 630 Agriculture

ISSN:

2352-7714

Publisher:

Elsevier

Language:

English

Submitter:

Simone Schuller

Date Deposited:

29 Oct 2021 15:54

Last Modified:

31 Oct 2021 01:42

Publisher DOI:

10.1016/j.onehlt.2021.100322

PubMed ID:

34522760

Uncontrolled Keywords:

3GC-R, Third Generation Cephalosporin-resistant 3GC-R-Ent., Third Generation Cephalosporin-resistant Enterobacterales AMR, Antimicrobial resistance CI, Confidence interval CLSI, Clinical and Laboratory Standards Institute COL-R, Colistin-resistant CP, Carbapenemase-producing CR, Carbapenem-resistant CRE, Carbapenem-resistant Enterobacterales Carbapenemase-producing Enterobacterales Co-carriage Companion animal ERIC-PCR, Enterobacterial repetitive intergenic consensus polymerase chain reaction ESBL, Extended spectrum β-lactamase ESBL-E. coli, ESBL-producing Escherichia coli ESBL-KP, ESBL-producing Klebsiella pneumoniae EUCAST, European Committee on Antimicrobial Susceptibility Testing Extended-spectrum β-lactamase IQR, Interquartile range KP, Klebsiella pneumoniae MALDI-TOF MS, Matrix-assisted laser desorption/ionization time of flight mass spectrometry MDR, Multidrug-resistant MDROs, Multidrug-resistant organisms MICs, Minimal inhibitory concentrations MLST, Multilocus sequence typing MR, Methicillin-resistant MRCoNS, Methicillin-resistant coagulase-negative staphylococci MRSA, Methicillin-resistant Staphylococcus aureus MRSP, Methicillin-resistant Staphylococcus pseudintermedius REP-PCR, Repetitive element palindromic polymerase chain reaction ST, Sequence type TMP-S, Trimethoprim/sulfamethoxazole Transmission WGS, Whole-genome sequencing pAmpC, Plasmid-encoded AmpC

BORIS DOI:

10.48350/160126

URI:

https://boris.unibe.ch/id/eprint/160126

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