Absolute quantification of transcription factors in human erythropoiesis using selected reaction monitoring mass spectrometry.

Gillespie, Mark A.; Palii, Carmen G.; Sanchez-Taltavull, Daniel; Perkins, Theodore J.; Brand, Marjorie; Ranish, Jeffrey A. (2020). Absolute quantification of transcription factors in human erythropoiesis using selected reaction monitoring mass spectrometry. STAR protocols, 1(3), p. 100216. Elsevier 10.1016/j.xpro.2020.100216

Preview

Text main__3_.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (3MB) | Preview

Quantitative changes in transcription factor (TF) abundance regulate dynamic cellular processes, including cell fate decisions. Protein copy number provides information about the relative stoichiometry of TFs that can be used to determine how quantitative changes in TF abundance influence gene regulatory networks. In this protocol, we describe a targeted selected reaction monitoring (SRM)-based mass-spectrometry method to systematically measure the absolute protein concentration of nuclear TFs as human hematopoietic stem and progenitor cells differentiate along the erythropoietic lineage. For complete details on the use and execution of this protocol, please refer to Gillespie et al. (2020).

Interest & Impact

Downloads

79 since deposited on 26 Oct 2021
25 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item