Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.

Zimmermann, Jakob (2021). Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation. Methods in molecular biology, 2285, pp. 111-119. Springer 10.1007/978-1-0716-1311-5_9

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An important hallmark for the characterisation of Th cells is their capacity for cytokine expression. In this chapter, we describe how Th cells can be restimulated polyclonally to reveal their cytokine-producing potential that can then be analysed by intracellular staining and flow cytometry.

Item Type:

 Journal Article (Further Contribution)

Division/Institute:

 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

UniBE Contributor:

 Zimmermann, Jakob

Subjects:

 600 Technology > 610 Medicine & health

ISSN:

 1940-6029

Publisher:

 Springer

Language:

 English

Submitter:

 Rahel Fuhrer

Date Deposited:

 08 Nov 2021 16:21

Last Modified:

 05 Dec 2022 15:54

Publisher DOI:

 10.1007/978-1-0716-1311-5_9

PubMed ID:

 33928547

Uncontrolled Keywords:

 Cytokines Flow cytometry Intracellular staining PMA/ionomycin T cell activation Th cells

BORIS DOI:

 10.48350/160618

URI:

 https://boris.unibe.ch/id/eprint/160618

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