Good agreement between capillary and venous sampling for lectin pathway proteins

Schlapbach, Luregn J.; Woerner, Andreas; Thiel, Steffen; Ammann, Roland A.; Aebi, Christoph; Nelle, Mathias; Jensenius, Jens C. (2013). Good agreement between capillary and venous sampling for lectin pathway proteins. Immunobiology, 218(4), pp. 465-469. Amsterdam: Elsevier 10.1016/j.imbio.2012.06.001

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Background: The lectin pathway of complement activation, in particular mannose-binding lectin (MBL), has been extensively investigated over recent years. So far, studies were exclusively based on venous samples. The aim of this study was to investigate whether measurements of lectin pathway proteins obtained by capillary sampling are in agreement with venous samples.

Methods: Prospective study including 31 infants that were admitted with suspected early-onset sepsis. Lectin pathway proteins were measured in simultaneously obtained capillary and venous samples. Bland–Altman plots of logarithmized results were constructed, and the mean capillary to venous ratios (ratiocap/ven) were calculated with their 95% confidence intervals (CI).
Results: The agreement between capillary and venous sampling was very high for MBL (mean ratiocap/ven, 1.01; 95% CI, 0.85–1.19). Similarly, high agreement was observed for H-ficolin (mean ratiocap/ven, 1.02; 95% CI, 0.72–1.44), MASP-2 (1.04; 0.59–1.84), MASP-3 (0.96; 0.71–1.28), and MAp44 (1.01; 0.82–1.25), while the agreement was moderate for M-ficolin (mean ratiocap/ven, 0.78; 95% CI, 0.27–2.28).

Conclusions: The results of this study show an excellent agreement between capillary and venous samples for most lectin pathway proteins. Except for M-ficolin, small volume capillary samples can thus be used when assessing lectin pathway proteins in neonates and young children.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
UniBE Contributor:	Schlapbach, Luregn Jan, Ammann, Roland, Aebi, Christoph, Nelle, Mathias
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health
ISSN:	0171-2985
Publisher:	Elsevier
Language:	English
Submitter:	Anette van Dorland
Date Deposited:	04 Oct 2013 14:40
Last Modified:	05 Dec 2022 14:12
Publisher DOI:	10.1016/j.imbio.2012.06.001
PubMed ID:	22749981
Web of Science ID:	000319024100004
URI:	https://boris.unibe.ch/id/eprint/16102 (FactScience: 223678)