Romano, Mariele; Bravin, Dr. Alberto; Wright, Dr. Michael D.; Jacques, Laurent; Miettinen, Dr. Arttu; Hlushchuk, Ruslan; Dinkel, Julien; Bartzsch, Dr. Stefan; Laissue, Jean Albert; Djonov, Valentin; Coan, Dr. Paola (2022). X-ray Phase Contrast 3D virtual histology: evaluation of lung alterations after micro-beam irradiation. International journal of radiation oncology, biology, physics, 112(3), pp. 818-830. Elsevier 10.1016/j.ijrobp.2021.10.009
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Purpose: This study provides the first experimental application of multiscale three-dimensional (3D) X-ray Phase Contrast Imaging Computed Tomography (XPCI-CT) virtual histology for the inspection and quantitative assessment of the late stage effects of radio-induced lesions on lungs in a small animal model.
Methods and materials: Healthy male Fischer rats were irradiated with X-ray standard broad beams and Microbeam Radiation Therapy (MRT), a high dose rate (14 kGy/s), FLASH spatially-fractionated X-ray therapy to avoid the beamlets smearing due to cardiosynchronous movements of the organs during the irradiation. After organ dissection, ex-vivo XPCI-CT was applied to all the samples and the results were quantitatively analysed and correlated to histologic data.
Results: XPCI-CT enables the 3D visualization of lung tissues with unprecedented contrast and sensitivity allowing alveoli, vessels and bronchi hierarchical visualization. XPCI-CT discriminates in 3D radio-induced lesions such as fibrotic scars, Ca/Fe deposits and, in addition, allows a full-organ accurate quantification of the fibrotic tissue within the irradiated organs. The radiation-induced fibrotic tissue content is less than 10% of the analyzed volume for all the MRT treated organs while it reaches the 34% in the case of irradiations with 50 Gy using a broad beam.
Conclusions: XPCI-CT is an effective imaging technique able to provide detailed 3D information for the assessment of lung pathology and treatment efficacy in a small animal model.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy |
UniBE Contributor: |
Hlushchuk, Ruslan, Laissue, Jean, Djonov, Valentin Georgiev |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0360-3016 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
David Christian Haberthür |
Date Deposited: |
01 Dec 2021 11:36 |
Last Modified: |
05 Dec 2022 15:54 |
Publisher DOI: |
10.1016/j.ijrobp.2021.10.009 |
PubMed ID: |
34678432 |
BORIS DOI: |
10.48350/161282 |
URI: |
https://boris.unibe.ch/id/eprint/161282 |
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