Insights into the Phytochemical and Multifunctional Biological Profile of Spices from the Genus Piper.

Luca, Simon Vlad; Gaweł-Bęben, Katarzyna; Strzępek-Gomółka, Marcelina; Czech, Karolina; Trifan, Adriana; Zengin, Gokhan; Korona-Glowniak, Izabela; Minceva, Mirjana; Gertsch, Jürg; Skalicka-Woźniak, Krystyna (2021). Insights into the Phytochemical and Multifunctional Biological Profile of Spices from the Genus Piper. Antioxidants, 10(10) MDPI 10.3390/antiox10101642

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Piper spices represent an inexhaustible reservoir of bioactive compounds that may act as drug leads in natural product research. The aim of this study was to investigate a series of methanolic fruit extracts obtained from P. nigrum (black, green, white and red), P. longum and P. retrofractum in comparative phytochemical and multi-directional biological (antimicrobial, antioxidant, anti-enzymatic and anti-melanogenic) assays. The metabolite profiling revealed the presence of 17 piperamides, with a total content of 247.75-591.42 mg piperine equivalents/g. Among the 22 tested microorganism strains, Piper spices were significantly active (MIC < 0.1 mg/mL) against the anaerobes Actinomyces israelii and Fusobacterium nucleatum. The antioxidant and anti-enzymatic activities were evidenced in DPPH (10.64-82.44 mg TE/g) and ABTS (14.20-77.60 mg TE/g) radical scavenging, CUPRAC (39.94-140.52 mg TE/g), FRAP (16.05-77.00 mg TE/g), chelating (0-34.80 mg EDTAE/g), anti-acetylcholinesterase (0-2.27 mg GALAE/g), anti-butyrylcholinesterase (0.60-3.11 mg GALAE/g), anti-amylase (0.62-1.11 mmol ACAE/g) and anti-glucosidase (0-1.22 mmol ACAE/g) assays. Several Piper extracts (10 μg/mL) inhibited both melanin synthesis (to 32.05-60.65% of αMSH+ cells) and release (38.06-45.78% of αMSH+ cells) in αMSH-stimulated B16F10 cells, partly explained by their tyrosinase inhibitory properties. Our study uncovers differences between Piper spices and sheds light on their potential use as nutraceuticals or cosmeceuticals for the management of different diseases linked to bacterial infections, Alzheimer's dementia, type 2 diabetes mellitus or hyperpigmentation.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Gertsch, Jürg


500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health








Barbara Franziska Järmann-Bangerter

Date Deposited:

02 Dec 2021 09:38

Last Modified:

02 Dec 2021 09:38

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Piper anti-melanogenic anti-tyrosinase antimicrobial natural bioactive agents piperamides




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