Ayer, Maxime; Burri, Olivier; Guiet, Romain; Seitz, Arne; Kaba, Elisa; Engelhardt, Britta; Klok, Harm-Anton (2021). Biotin-NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes. Bioconjugate chemistry, 32(3), pp. 541-552. American Chemical Society 10.1021/acs.bioconjchem.1c00026
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Cells are powerful carriers that can help to improve the delivery of nanomedicines. One approach to use cells as carriers is to immobilize the nanoparticulate cargo on the cell surface. While a plethora of chemical conjugation strategies are available to bind nanoparticles to cell surfaces, only relatively little is known about the effects of particle size and cell type on the surface immobilization of nanoparticles. This study investigates the biotin-NeutrAvidin mediated immobilization of model polymer nanoparticles with sizes ranging from 40 nm to 1 μm on two different T cell lines, viz., human Jurkat cells as well as mouse SJL/PLP7 T cells, which are of potential interest for drug delivery across the blood-brain barrier. The nanoparticle cell surface immobilization and the particle surface concentration and distribution were analyzed by flow cytometry and confocal microscopy. The functional properties of nanoparticle-modified SJL/PLP7 T cells were assessed in an ICAM-1 binding assay as well as in a two-chamber setup in which the migration of the particle-modified T cells across an in vitro model of the blood-brain barrier was studied. The results of these experiments highlight the effects of particle size and cell line on the surface immobilization of nanoparticles on living cells.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
UniBE Contributor: |
Bouillet, Elisa Catherine, Engelhardt, Britta |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1043-1802 |
Publisher: |
American Chemical Society |
Language: |
English |
Submitter: |
Cindy Carolyn Alpinice Schumacher |
Date Deposited: |
21 Dec 2021 11:34 |
Last Modified: |
09 Jul 2023 03:26 |
Publisher DOI: |
10.1021/acs.bioconjchem.1c00026 |
PubMed ID: |
33621057 |
BORIS DOI: |
10.48350/162849 |
URI: |
https://boris.unibe.ch/id/eprint/162849 |
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