A mixed chirality α-helix in a stapled bicyclic and a linear antimicrobial peptide revealed by X-ray crystallography

Baeriswyl, Stéphane; Personne, Hippolyte; Di Bonaventura, Ivan; Köhler, Thilo; van Delden, Christian; Stocker, Achim; Javor, Sacha; Reymond, Jean-Louis (2021). A mixed chirality α-helix in a stapled bicyclic and a linear antimicrobial peptide revealed by X-ray crystallography. RSC chemical biology, 2(6), pp. 1608-1617. Royal Society of Chemistry 10.1039/D1CB00124H

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The peptide α-helix is right-handed when containing amino acids with L-chirality, and left-handed with D-chirality, however mixed chirality peptides generally do not form α-helices unless a helix inducer such as the non-natural residue amino-isobutyric acid is used. Herein we report the first X-ray crystal structures of mixed chirality α-helices in short peptides comprising only natural residues as the example of a stapled bicyclic and a linear membrane disruptive amphiphilic antimicrobial peptide (AMP) containing seven L- and four D-residues, as complexes of fucosylated analogs with the bacterial lectin LecB. The mixed chirality α-helices are superimposable onto the homochiral α-helices and form under similar conditions as shown by CD spectra and MD simulations but non-hemolytic and resistant to proteolysis. The observation of a mixed chirality α-helix with only natural residues in the protein environment of LecB suggests a vast unexplored territory of α-helical mixed chirality sequences and their possible use for optimizing bioactive α-helical peptides.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Personne, Hippolyte; Stocker, Achim; Javor, Sacha and Reymond, Jean-Louis

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

2633-0679

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Sandra Tanja Zbinden Di Biase

Date Deposited:

19 Jan 2022 15:42

Last Modified:

05 Dec 2022 15:59

Publisher DOI:

10.1039/D1CB00124H

PubMed ID:

34977576

BORIS DOI:

10.48350/163012

URI:

https://boris.unibe.ch/id/eprint/163012

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