Liu, Yuanhui; Ma, Bin; Chen, Wenfeng; Schlaeppi, Klaus; Erb, Matthias; Stirling, Erinne; Hu, Lingfei; Wang, Entao; Zhang, Yunzeng; Zhao, Kankan; Lu, Zhijiang; Ye, Shudi; Xu, Jianming (2021). Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean. Frontiers in Microbiology, 12, p. 709012. Frontiers 10.3389/fmicb.2021.709012
|
Text
2021_FrontMicrobiol_12_709012.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (4MB) | Preview |
Root-microbiome interactions are of central importance for plant performance and yield. A distinctive feature of legumes is that they engage in symbiosis with N-2-fixing rhizobia. If and how the rhizobial symbiotic capacity modulates root-associated microbiomes are still not yet well understood. We determined root-associated microbiomes of soybean inoculated with wild type (WT) or a noeI mutant of Bradyrhizobium diazoefficiens USDA 110 by amplicon sequencing. UPLC-MS/MS was used to analyze root exudates. The noeI gene is responsible for fucose-methylation of Nod factor secreted by USDA 110 WT strain. Soybean roots inoculated with the noeI mutant showed a significant decrease in nodulation and root-flavonoid exudation compared to roots inoculated with WT strain. The noeI mutant-inoculated roots exhibited strong changes in microbiome assembly in the rhizosphere and rhizoplane, including reduced diversity, changed co-occurrence interactions and a substantial depletion of root microbes. Root exudates and soil physiochemical properties were significantly correlated with microbial community shift in the rhizosphere between different rhizobial treatments. These results illustrate that rhizobial symbiotic capacity dramatically alters root-associated microbiomes, in which root exudation and edaphic patterns play a vital role. This study has important implications for understanding the evolution of plant-microbiome interactions.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Schläppi, Klaus Bernhard, Erb, Matthias, Hu, Lingfei |
Subjects: |
500 Science > 580 Plants (Botany) |
ISSN: |
1664-302X |
Publisher: |
Frontiers |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
27 Jan 2022 10:57 |
Last Modified: |
05 Dec 2022 15:59 |
Publisher DOI: |
10.3389/fmicb.2021.709012 |
PubMed ID: |
34925249 |
Uncontrolled Keywords: |
root-associated microbiome, symbiosis, rhizobia, Glycine max, root exudation |
BORIS DOI: |
10.48350/163047 |
URI: |
https://boris.unibe.ch/id/eprint/163047 |
Actions (login required)
 |
Edit item |