The endocrine milieu in naturally matured follicles is different in women with high serum anti-Müllerian hormone concentrations.

von Wolff, Michael; Mitter, Vera Ruth; Jamir, Nadia; Stute, Petra; Eisenhut, Markus; Bersinger, Nick A. (2021). The endocrine milieu in naturally matured follicles is different in women with high serum anti-Müllerian hormone concentrations. Reproductive biomedicine online, 43(2), pp. 329-337. Elsevier 10.1016/j.rbmo.2021.01.021

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Is the endocrine milieu different in women with low serum anti-Müllerian hormone (AMH) concentration compared with women with high concentration?


Cohort study of 84 women (four groups) classified according to AMH concentration and age undergoing natural cycle IVF treatment. Concentrations of LH, oestradiol, testosterone, androstenedione and AMH were determined in follicular fluid (FF), associations analysed and clinical outcome parameters evaluated.


A positive correlation between serum and FF AMH concentrations was confirmed. Follicular fluid androstenedione concentration was positively correlated with serum AMH concentration (P < 0.0001, r2 = 0.197). The correlation between FF LH and FF testosterone concentration in all women was not significant (P = 0.050, r2 = 0.046); however, the correlation between FF androstenedione in women with high serum AMH concentration was significant (P = 0.032, r2 = 0.220). Follicular fluid testosterone and androstenedione were positively correlated with FF oestradiol overall and in some individual groups. The high serum AMH concentration group showed the highest FF AMH and androstenedione concentrations and lowest oestradiol-testosterone and oestradiol-androstenedione ratios. High FF AMH concentration was associated with a higher clinical pregnancy rate and high FF oestradiol concentration with a slightly better embryo quality.


Differences in the endocrine milieu in women with high serum AMH concentration seem to be caused by increased follicular LH concentration. In women with high serum AMH concentration, FF androstenedione is increased and ratios of oestradiol-testosterone and oestradiol-androstenedione are decreased, suggesting a disturbed endocrine milieu caused by reduced metabolization of FF androgens into oestrogens. In natural cycles, FF AMH concentrations are positively associated with higher clinical pregnancy rates and oestradiol concentrations with a higher embryo score.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

von Wolff, Michael, Mitter, Vera Ruth, Stute, Petra, Eisenhut, Markus, Bersinger, Nick A.


600 Technology > 610 Medicine & health








Monika Zehr

Date Deposited:

27 Dec 2021 15:09

Last Modified:

05 Dec 2022 15:59

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

AMH Assisted reproductive technologies Follicular fluid Follicular hormones Natural cycle IVF




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