Nishihara, Hideaki; Gastfriend, Benjamin D; Kasap, Pelin; Palecek, Sean P; Shusta, Eric V; Engelhardt, Britta (2021). Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions. STAR protocols, 2(2), p. 100563. Elsevier 10.1016/j.xpro.2021.100563
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We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion. For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a).
Item Type: |
Journal Article (Further Contribution) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Nishihara, Hideaki, Kasap, Pelin, Engelhardt, Britta |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2666-1667 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Cindy Carolyn Alpinice Schumacher |
Date Deposited: |
27 Dec 2021 09:32 |
Last Modified: |
23 Mar 2023 23:26 |
Publisher DOI: |
10.1016/j.xpro.2021.100563 |
PubMed ID: |
34151293 |
Uncontrolled Keywords: |
Cell Differentiation Cell culture Immunology Neuroscience Stem Cells |
BORIS DOI: |
10.48350/163158 |
URI: |
https://boris.unibe.ch/id/eprint/163158 |
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