Tampakis, Athanasios; Weixler, Benjamin; Rast, Silvan; Tampaki, Ekaterini-Christina; Cremonesi, Eleonora; Kancherla, Venkatesh; Tosti, Nadia; Kettelhack, Christoph; Ng, Charlotte K Y; Delko, Tarik; Soysal, Savas D; von Holzen, Urs; Felekouras, Evangelos; Nikiteas, Nikolaos; Bolli, Martin; Tornillo, Luigi; Terracciano, Luigi; Eppenberger-Castori, Serenella; Spagnoli, Giulio C; Piscuoglio, Salvatore; ... (2021). Nestin and CD34 expression in colorectal cancer predicts improved overall survival. Acta oncologica, 60(6), pp. 727-734. Taylor & Francis 10.1080/0284186X.2021.1891280
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BACKGROUND
Nestin, a class VI intermediate filament protein of the cytoskeleton, and CD34, a transmembrane phosphoglycoprotein, are markers of progenitor cells. This study aimed to evaluate their expression and clinical significance in colorectal cancer.
METHODS
A clinically annotated tissue microarray, including 599 patients with colorectal cancer, was analyzed by immunohistochemistry. Furthermore, nestin and CD34 correlations with HIF-1a and a panel of cytokines and chemokines were assessed using quantitative reverse transcription PCR and The Cancer Genome Atlas dataset.
RESULTS
Expression of nestin and CD34 was observed only in the tumor stroma. Patients displaying high expression of nestin and CD34 demonstrated higher rates of T1 and T2 tumors (p = .020), lower vascular invasion (p < .001) and improved 5-year overall survival (65%; 95% CI = 55-73 vs 45%; 95% CI = 37-53) after adjusting for clinicopathological characteristics (HR: 0.67; 95% CI = 0.46-0.96). A moderate to strong correlation (r = 0.37-0.78, p < .03) of nestin and CD34 was demonstrated for the following markers; HIF-1α, CD4, CD8, FOXP3, IRF1, GATA3, CCL2, CCL3, CXCL12 and CCL21.
CONCLUSIONS
Combined expression of nestin and CD34 expression is associated with better overall survival possibly by modulating a favorable immune response.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
UniBE Contributor: |
Ng, Kiu Yan Charlotte |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1651-226X |
Publisher: |
Taylor & Francis |
Language: |
English |
Submitter: |
Marla Rittiner |
Date Deposited: |
21 Jan 2022 15:10 |
Last Modified: |
05 Dec 2022 16:00 |
Publisher DOI: |
10.1080/0284186X.2021.1891280 |
PubMed ID: |
33734917 |
Uncontrolled Keywords: |
CD34 Nestin colorectal cancer hypoxia associated immune response tumor microenvironment |
BORIS DOI: |
10.48350/163422 |
URI: |
https://boris.unibe.ch/id/eprint/163422 |
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