Impact of Cross-Linking of Collagen Matrices on Tissue Regeneration in a Rabbit Calvarial Bone Defect.

Fujioka-Kobayashi, Masako; Andrejova, Elena; Katagiri, Hiroki; Schaller, Benoit; Sculean, Anton; Imber, Jean-Claude; Lang, Niklaus P.; Saulacic, Nikola (2021). Impact of Cross-Linking of Collagen Matrices on Tissue Regeneration in a Rabbit Calvarial Bone Defect. Materials, 14(13) Molecular Diversity Preservation International MDPI 10.3390/ma14133740

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The cross-linking of collagen matrices (Cl_CM) may provide volume-stable enhanced defect regeneration when compared to non-cross-linked matrices (Ncl_CM). The aim of the present study was to investigate the bone forming potential of collagen matrices (CMs) and the effects of cross-linking CMs in a rabbit calvaria defect model. (1) Empty controls (n = 6), (2) Ncl_CM (n = 8), and (3) Cl_CM (n = 8) were selected to be observed for the healing in 10 mm critical-sized calvarial bone defects. The potential for the bone as well as the connective tissue formation were evaluated by micro-CT and histomorphometry at three months post-surgery. There were no statistically significant differences in terms of new bone volume in the defects between the groups. However, the Cl_CM induced significantly greater fibrous tissue regeneration (5.29 ± 1.57 mm2) when compared to the controls (3.51 ± 0.93 mm2) by histomorphometry. The remnants of collagen fibers with immune cells, including macrophages and giant cells, were occasionally observed in the Cl_CM group but not in the Ncl_CM group. In conclusion, the cross-linking of collagen did not influence the potential for bone formation. Nevertheless, Cl_CM might be advantageous for the maintenance of fibrous tissue volume without disturbing bone formation in the defects.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Katagiri, Hiroki; Schaller, Benoît; Sculean, Anton; Imber, Jean-Claude; Lang, Niklaus Peter and Saulacic, Nikola

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1996-1944

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

14 Jan 2022 13:20

Last Modified:

14 Jan 2022 13:26

Publisher DOI:

10.3390/ma14133740

PubMed ID:

34279311

Uncontrolled Keywords:

bone formation collagen cross-linking tissue augmentation

BORIS DOI:

10.48350/163494

URI:

https://boris.unibe.ch/id/eprint/163494

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