Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study.

Katagiri, Hiroki; El Tawil, Yacine; Lang, Niklaus P.; Imber, Jean-Claude; Sculean, Anton; Kobayashi, Masako; Saulacic, Nikola (2021). Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study. Biomedicines, 9(2) MDPI 10.3390/biomedicines9020143

biomedicines-09-00143-v2.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (3MB) | Preview

The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Katagiri, Hiroki; Lang, Niklaus Peter; Kobayashi, Masako and Saulacic, Nikola


600 Technology > 610 Medicine & health








Caroline Dominique Zürcher

Date Deposited:

04 Jan 2022 16:02

Last Modified:

04 Jan 2022 16:11

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

animal experiment collagen critical size defect histological analysis hydroxyapatite micro-CT




Actions (login required)

Edit item Edit item
Provide Feedback