Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold.

Imber, Jean-Claude; Roccuzzo, Andrea; Stähli, Alexandra; Saulacic, Nikola; Deschner, James; Sculean, Anton; Bosshardt, Dieter Daniel (2021). Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold. International journal of molecular sciences, 22(20) MDPI 10.3390/ijms222010915

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(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, p = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (p = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Imber, Jean-Claude; Roccuzzo, Andrea; Stähli, Alexandra Beatrice; Saulacic, Nikola; Sculean, Anton and Bosshardt, Dieter


600 Technology > 610 Medicine & health








Caroline Dominique Zürcher

Date Deposited:

17 Jan 2022 16:07

Last Modified:

17 Jan 2022 16:15

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

biomaterial collagen scaffold histology immunohistochemistry intrabony defect periodontal regeneration volume-stable collagen matrix




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