Cross-linked hyaluronic acid slows down collagen membrane resorption in diabetic rats through reducing the number of macrophages.

Eliezer, Meizi; Sculean, Anton; Miron, Richard J; Nemcovsky, Carlos; Bosshardt, Dieter D; Kobayashi, Masako; Weinreb, Miron; Moses, Ofer (2022). Cross-linked hyaluronic acid slows down collagen membrane resorption in diabetic rats through reducing the number of macrophages. Clinical oral investigations, 26(3), pp. 2401-2411. Springer 10.1007/s00784-021-04206-x

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OBJECTIVES

We previously showed that accelerated degradation of collagen membranes (CMs) in diabetic rats is associated with increased infiltration of macrophages and blood vessels. Since pre-implantation immersion of CMs in cross-linked high molecular weight hyaluronic acid (CLHA) delays membrane degradation, we evaluated here its effect on the number of macrophages and endothelial cells (ECs) within the CM as a possible mechanism for inhibition of CM resorption.

MATERIALS AND METHODS

Diabetes was induced with streptozotocin in 16 rats, while 16 healthy rats served as control. CM discs were labeled with biotin, soaked in CLHA or PBS, and implanted under the scalp. Fourteen days later, CMs were embedded in paraffin and the number of macrophages and ECs within the CMs was determined using antibodies against CD68 and transglutaminase II, respectively.

RESULTS

Diabetes increased the number of macrophages and ECs within the CMs (∼2.5-fold and fourfold, respectively). Immersion of CMs in CLHA statistically significantly reduced the number of macrophages (p < 0.0001) in diabetic rats, but not that of ECs. In the healthy group, CLHA had no significant effect on the number of either cells. Higher residual collagen area and membrane thickness in CLHA-treated CMs in diabetic animals were significantly correlated with reduced number of macrophages but not ECs.

CONCLUSIONS

Immersion of CM in CLHA inhibits macrophage infiltration and reduces CM degradation in diabetic animals.

CLINICAL RELEVANCE

The combination of CLHA and CM may represent a valuable approach when guided tissue regeneration or guided bone regeneration procedures are performed in diabetic patients.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

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04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Eliezer-Shatz, Meizi, Sculean, Anton, Miron, Richard John, Bosshardt, Dieter, Kobayashi, Masako (B)

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1436-3771

Publisher:

Springer

Language:

English

Submitter:

Doris Burri

Date Deposited:

27 Jan 2022 09:38

Last Modified:

29 Mar 2023 23:38

Publisher DOI:

10.1007/s00784-021-04206-x

PubMed ID:

34608575

Uncontrolled Keywords:

Blood vessels Collagen membranes Diabetes Hyaluronic acid Macrophages Rats

BORIS DOI:

10.48350/163837

URI:

https://boris.unibe.ch/id/eprint/163837

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