Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and Tissues.

Rath-Deschner, Birgit; Nogueira, Andressa Vilas Boas; Memmert, Svenja; Nokhbehsaim, Marjan; Augusto Cirelli, Joni; Eick, Sigrun; Miosge, Nicolai; Kirschneck, Christian; Kesting, Marco; Deschner, James; Jäger, Andreas; Damanaki, Anna (2021). Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and Tissues. International journal of molecular sciences, 22(2) MDPI 10.3390/ijms22020591

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The aim of the study was to clarify whether orthodontic forces and periodontitis interact with respect to the anti-apoptotic molecules superoxide dismutase 2 (SOD2) and baculoviral IAP repeat-containing protein 3 (BIRC3). SOD2, BIRC3, and the apoptotic markers caspases 3 (CASP3) and 9 (CASP9) were analyzed in gingiva from periodontally healthy and periodontitis subjects by real-time PCR and immunohistochemistry. SOD2 and BIRC3 were also studied in gingiva from rats with experimental periodontitis and/or orthodontic tooth movement. Additionally, SOD2 and BIRC3 levels were examined in human periodontal fibroblasts incubated with Fusobacterium nucleatum and/or subjected to mechanical forces. Gingiva from periodontitis patients showed significantly higher SOD2, BIRC3, CASP3, and CASP9 levels than periodontally healthy gingiva. SOD2 and BIRC3 expressions were also significantly increased in the gingiva from rats with experimental periodontitis, but the upregulation of both molecules was significantly diminished in the concomitant presence of orthodontic tooth movement. In vitro, SOD2 and BIRC3 levels were significantly increased by F. nucleatum, but this stimulatory effect was also significantly inhibited by mechanical forces. Our study suggests that SOD2 and BIRC3 are produced in periodontal infection as a protective mechanism against exaggerated apoptosis. In the concomitant presence of orthodontic forces, this protective anti-apoptotic mechanism may get lost.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1422-0067

Publisher:

MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

26 Jan 2022 15:32

Last Modified:

26 Jan 2022 15:32

Publisher DOI:

10.3390/ijms22020591

PubMed ID:

33435582

Uncontrolled Keywords:

Fusobacterium nucleatum gingivitis orthodontic tooth movement periodontal ligament periodontitis periodontium

BORIS DOI:

10.48350/163916

URI:

https://boris.unibe.ch/id/eprint/163916

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