Panahipour, Layla; Omerbasic, Anes; Nasirzade, Jila; Gruber, Reinhard (2021). TGF-β Activity of a Demineralized Bone Matrix. International journal of molecular sciences, 22(2) MDPI 10.3390/ijms22020664
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Allografts consisting of demineralized bone matrix (DBM) are supposed to retain the growth factors of native bone. However, it is not clear if transforming growth factor β1 (TGF-β1) is maintained in the acid-extracted human bone. To this aim, the aqueous solutions of supernatants and acid lysates of OraGRAFT® Demineralized Cortical Particulate and OraGRAFT® Prime were prepared. Exposing fibroblasts to the aqueous solution caused a TGF-β receptor type I kinase-inhibitor SB431542-dependent increase in interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) expression. Interleukin 11 expression and the presence of TGF-β1 in the aqueous solutions were confirmed by immunoassay. Immunofluorescence further confirmed the nuclear translocation of Smad2/3 when fibroblasts were exposed to the aqueous solutions of both allografts. Moreover, allografts released matrix metalloprotease-2 activity and blocking proteases diminished the cellular TGF-β response to the supernatant. These results suggest that TGF-β is preserved upon the processing of OraGRAFT® and released by proteolytic activity into the aqueous solution.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
26 Jan 2022 15:40 |
Last Modified: |
05 Dec 2022 16:01 |
Publisher DOI: |
10.3390/ijms22020664 |
PubMed ID: |
33440877 |
Uncontrolled Keywords: |
allografts bioassay bone augmentation bone regeneration demineralized bone matrix transforming growth factor β1 |
BORIS DOI: |
10.48350/163941 |
URI: |
https://boris.unibe.ch/id/eprint/163941 |
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