Strauss, Franz-Josef; Kuchler, Ulrike; Kobatake, Reiko; Heimel, Patrick; Tangl, Stefan; Gruber, Reinhard (2021). Acid bone lysates reduce bone regeneration in rat calvaria defects. Journal of biomedical materials research. Part A, 109(5), pp. 659-665. Wiley 10.1002/jbm.a.37050
|
Text
J_Biomedical_Materials_Res_-_2020_-_Strauss_-_Acid_bone_lysates_reduce_bone_regeneration_in_rat_calvaria_defects.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (4MB) | Preview |
Acid bone lysates (ABLs) represent the growth factors and other molecules released during autologous graft resorption. However, the impact of these bone-derived growth factors on the healing of bone defects has not yet been investigated. The aim of the present study was, therefore, to examine the impact of ABLs adsorbed to collagen membranes on bone regeneration. To this end, in 16 female Sprague Dawley rats, a standardized 5-mm-diameter critical size defect on the calvarial bone was created. The defects were covered with collagen membranes that had been soaked either in serum-free media or ABLs followed by lyophilization. After a healing period of 4 weeks, micro-computed tomography (μCT) and histological analyses by means of undecalcified thin ground sections were performed. μCT analysis of the inner 4 mm of the calvaria defect showed a greater bone defect coverage in the control group when compared to ABL group, 29.8% (confidence interval [CI]: 17.7-50.3) versus 5.6% (CI: 1.0-29.8, p = .03), respectively. Moreover, we found significantly more absolute bone volume (BV) in the control group when compared to ABL group, 0.59 mm3 (CI: 0.27-1.25) versus 0.07 mm3 (CI: 0.06-0.59, p = .04), respectively. Histomorphometry confirmed these findings with a relative BV in the central compartment of 14.1% (CI: 8.4-20.6) versus 5.6% (CI: 3.4-7.9, p = .004), respectively. These findings indicate that bone-derived growth factors contained in ABLs are able to attenuate bone regeneration within collagen membranes.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1552-4965 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
27 Jan 2022 11:49 |
Last Modified: |
05 Dec 2022 16:01 |
Publisher DOI: |
10.1002/jbm.a.37050 |
PubMed ID: |
32608132 |
Uncontrolled Keywords: |
bone allograft bone grafts bone regeneration growth factors |
BORIS DOI: |
10.48350/163942 |
URI: |
https://boris.unibe.ch/id/eprint/163942 |
Actions (login required)
 |
Edit item |