Panahipour, Layla; Moghaddam, Dariush Mehdipour; Nasirzade, Jila; Kargarpour, Zahra; Gruber, Reinhard (2021). RNAseq of TGF-β receptor type I kinase-dependent genes in oral fibroblast exposed to milk. BMC Oral Health, 21(1), p. 581. BioMed Central 10.1186/s12903-021-01913-5
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BACKGROUND
Milk is a rich source of natural growth factors that may support oral tissue homeostasis and wound healing. We had shown earlier that blocking TGF-β receptor type I kinase with the inhibitor SB431542 abolished the expression of IL11 and other genes in human gingival fibroblasts exposed to the aqueous fraction of milk. Our aim was to identify the entire signature of TGF-β receptor type I kinase-dependent genes regulated by the aqueous fraction of human milk.
RESULT
RNAseq revealed 99 genes being strongly regulated by milk requiring activation of the SB431542-dependent TGF-β receptor type I kinase. Among the SB431542-dependent genes is IL11 but also cadherins, claudins, collagens, potassium channels, keratins, solute carrier family proteins, transcription factors, transmembrane proteins, tumor necrosis factor ligand superfamily members, and tetraspanin family members. When focusing on our candidate gene, we could identify D609 to suppress IL11 expression, independent of phospholipase C, sphinosine-1 phosphate synthesis, and Smad-3 phosphorylation and its nuclear translocation. In contrast, genistein and blocking phosphoinositide 3-kinases by wortmannin and LY294002 increased the milk-induced IL11 expression in gingival fibroblasts.
CONCLUSION
Taken together, our data revealed TGF-β receptor type I kinase signaling to cause major changes of the genetic signature of gingival fibroblasts exposed to aqueous fraction of human milk.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1472-6831 |
Publisher: |
BioMed Central |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
26 Jan 2022 12:34 |
Last Modified: |
05 Dec 2022 16:01 |
Publisher DOI: |
10.1186/s12903-021-01913-5 |
PubMed ID: |
34789212 |
Uncontrolled Keywords: |
Gingival fibroblast Human milk In vitro TGF-β activity TGF-β receptor type I kinase |
BORIS DOI: |
10.48350/163954 |
URI: |
https://boris.unibe.ch/id/eprint/163954 |
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