Kargarpour, Zahra; Nasirzade, Jila; Panahipour, Layla; Mitulović, Goran; Miron, Richard J.; Gruber, Reinhard (2021). Platelet-Rich Fibrin Increases BMP2 Expression in Oral Fibroblasts via Activation of TGF-β Signaling. International journal of molecular sciences, 22(15) MDPI 10.3390/ijms22157935
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Solid platelet-rich fibrin (PRF), consisting of coagulated plasma from fractionated blood, has been proposed to be a suitable carrier for recombinant bone morphogenetic protein 2 (BMP2) to target mesenchymal cells during bone regeneration. However, whether solid PRF can increase the expression of BMPs in mesenchymal cells remains unknown. Proteomics analysis confirmed the presence of TGF-β1 but not BMP2 in PRF lysates. According to the existing knowledge of recombinant TGF-β1, we hypothesized that PRF can increase BMP2 expression in mesenchymal cells. To test this hypothesis, we blocked TGF-β receptor 1 kinase with SB431542 in gingival fibroblasts exposed to PRF lysates. RT-PCR and immunoassays confirmed that solid PRF lysates caused a robust SB431542-dependent increase in BMP2 expression in gingival fibroblasts. Additionally, fractions of liquid PRF, namely platelet-poor plasma (PPP) and the buffy coat (BC) layer, but not heat-denatured PPP (Alb-gel), greatly induced the expression of BMP2 in gingival fibroblasts. Even though PRF has no detectable BMPs, PRF lysates similar to recombinant TGF-β1 had the capacity to provoke canonical BMP signaling, as indicated by the nuclear translocation of Smad1/5 and the increase in its phosphorylation. Taken together, our data suggest that PRF can activate TGF-β receptor 1 kinase and consequently induce the production of BMP2 in cells of the mesenchymal lineage.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Miron, Richard John, Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
26 Jan 2022 15:48 |
Last Modified: |
01 Apr 2024 00:29 |
Publisher DOI: |
10.3390/ijms22157935 |
PubMed ID: |
34360701 |
Uncontrolled Keywords: |
BMP2 bone regeneration buffy coat platelet-poor plasma platelet-rich fibrin proteomics |
BORIS DOI: |
10.48350/163956 |
URI: |
https://boris.unibe.ch/id/eprint/163956 |
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