Nasirzade, Jila; Alccayhuaman, Karol Alí Apaza; Kargarpour, Zahra; Kuchler, Ulrike; Strauss, Franz Josef; Panahipour, Layla; Kampleitner, Carina; Heimel, Patrick; Schwarz, Frank; Gruber, Reinhard (2021). Acid Dentin Lysate Failed to Modulate Bone Formation in Rat Calvaria Defects. Biology, 10(3) MDPI 10.3390/biology10030196
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Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since tooth roots undergo creeping substitution similar to bone grafts, it can be hypothesized that osteoclasts release the growth factors stored in the dentin thereby influencing bone formation. To test this hypothesis, collagen membranes were either soaked in acid dentin lysates (ADL) from extracted porcine teeth or serum-free medium followed by lyophilization. Thereafter, these membranes covered standardized 5-mm-diameter critical-size defects in calvarial bone on rats. After four weeks of healing, micro-computed tomography and histological analyses using undecalcified thin ground sections were performed. Micro-computed tomography of the inner 4.5 mm calvaria defects revealed a median bone defect coverage of 91% (CI: 87-95) in the ADL group and 94% (CI: 65-100) in the control group, without significant differences between the groups (intergroup p > 0.05). Furthermore, bone volume (BV) was similar between ADL group (5.7 mm3, CI: 3.4-7.1) and control group (5.7 mm3, CI: 2.9-9.7). Histomorphometry of the defect area confirmed these findings with bone area values amounting to 2.1 mm2 (CI: 1.2-2.6) in the ADL group and 2.0 mm2 (CI: 1.1-3.0) in the control group. Together, these data suggest that acid dentin lysate lyophilized onto collagen membranes failed to modulate the robust bone formation when placed onto calvarial defects.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2079-7737 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
26 Jan 2022 12:14 |
Last Modified: |
05 Dec 2022 16:01 |
Publisher DOI: |
10.3390/biology10030196 |
PubMed ID: |
33807565 |
Uncontrolled Keywords: |
autogenous tooth roots bone augmentation bone regeneration collagen membranes guided bone regeneration rat calvaria defect |
BORIS DOI: |
10.48350/163959 |
URI: |
https://boris.unibe.ch/id/eprint/163959 |
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