Alccayhuaman, Karol Alí Apaza; Tangl, Stefan; Blouin, Stéphane; Hartmann, Markus A; Heimel, Patrick; Kuchler, Ulrike; Lee, Jung-Seok; Gruber, Reinhard (2021). Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study. Biomedicines, 9(7) MDPI 10.3390/biomedicines9070732
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Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm2 (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm2 (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm3 (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2227-9059 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
26 Jan 2022 12:23 |
Last Modified: |
01 Apr 2024 00:29 |
Publisher DOI: |
10.3390/biomedicines9070732 |
PubMed ID: |
34202317 |
Uncontrolled Keywords: |
bone regeneration collagen matrix histology qBEI volume-stable collagen matrix |
BORIS DOI: |
10.48350/163960 |
URI: |
https://boris.unibe.ch/id/eprint/163960 |
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