Vacchi, Elena; Burrello, Jacopo; Burrello, Alessio; Bolis, Sara; Monticone, Silvia; Barile, Lucio; Kaelin-Lang, Alain; Melli, Giorgia (2021). Profiling Inflammatory Extracellular Vesicles in Plasma and Cerebrospinal Fluid: An Optimized Diagnostic Model for Parkinson's Disease. Biomedicines, 9(3) MDPI 10.3390/biomedicines9030230
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Vacchi__2021__Profiling_Inflammatory_Extracellular_Vesicles.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (4MB) | Preview |
Extracellular vesicles (EVs) play a central role in intercellular communication, which is relevant for inflammatory and immune processes implicated in neurodegenerative disorders, such as Parkinson's Disease (PD). We characterized and compared distinctive cerebrospinal fluid (CSF)-derived EVs in PD and atypical parkinsonisms (AP), aiming to integrate a diagnostic model based on immune profiling of plasma-derived EVs via artificial intelligence. Plasma- and CSF-derived EVs were isolated from patients with PD, multiple system atrophy (MSA), AP with tauopathies (AP-Tau), and healthy controls. Expression levels of 37 EV surface markers were measured by a flow cytometric bead-based platform and a diagnostic model based on expression of EV surface markers was built by supervised learning algorithms. The PD group showed higher amount of CSF-derived EVs than other groups. Among the 17 EV surface markers differentially expressed in plasma, eight were expressed also in CSF of a subgroup of PD, 10 in MSA, and 6 in AP-Tau. A two-level random forest model was built using EV markers co-expressed in plasma and CSF. The model discriminated PD from non-PD patients with high sensitivity (96.6%) and accuracy (92.6%). EV surface marker characterization bolsters the relevance of inflammation in PD and it underscores the role of EVs as pathways/biomarkers for protein aggregation-related neurodegenerative diseases.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology |
UniBE Contributor: |
Kaelin, Alain |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2227-9059 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Chantal Kottler |
Date Deposited: |
01 Feb 2022 14:51 |
Last Modified: |
05 Dec 2022 16:02 |
Publisher DOI: |
10.3390/biomedicines9030230 |
PubMed ID: |
33669043 |
Uncontrolled Keywords: |
Parkinson’s disease biomarkers cerebrospinal fluid extracellular vesicles machine learning multiple system atrophy neuroinflammation plasma tauopathies |
BORIS DOI: |
10.48350/164096 |
URI: |
https://boris.unibe.ch/id/eprint/164096 |
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