Variation and Selection in the Putative Sperm-Binding Region of ZP3 in Muroid Rodents: A Comparison between Cricetids and Murines

Duarte, Margarida Alexandra; Rodríguez Fernandes, Carlos; Heckel, Gerald; da Luz Mathias, Maria; Bastos-Silveira, Cristiane (2021). Variation and Selection in the Putative Sperm-Binding Region of ZP3 in Muroid Rodents: A Comparison between Cricetids and Murines. Genes, 12(9), p. 1450. MDPI, Molecular Diversity Preservation International 10.3390/genes12091450

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In mammals, the zona pellucida glycoprotein 3 (ZP3) is considered a primary sperm receptor of the oocyte and is hypothesized to be involved in reproductive isolation. We investigated patterns of diversity and selection in the putative sperm-binding region (pSBR) of mouse ZP3 across Cricetidae and Murinae, two hyperdiverse taxonomic groups within muroid rodents. In murines, the pSBR is fairly conserved, in particular the serine-rich stretch containing the glycosylation sites proposed as essential for sperm binding. In contrast, cricetid amino acid sequences of the pSBR were much more variable and the serine-rich motif, typical of murines, was generally substantially modified. Overall, our results suggest a general lack of species specificity of the pSBR across the two muroid families. We document statistical evidence of positive selection acting on exons 6 and 7 of
ZP3 and identified several amino acid sites that are likely targets of selection, with most positively selected sites falling within or adjacent to the pSBR.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Population Genetics

UniBE Contributor:

Heckel, Gerald

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2073-4425

Publisher:

MDPI, Molecular Diversity Preservation International

Language:

English

Submitter:

Susanne Holenstein

Date Deposited:

01 Feb 2022 12:05

Last Modified:

23 Dec 2022 09:37

Publisher DOI:

10.3390/genes12091450

PubMed ID:

34573431

Uncontrolled Keywords:

zona pellucida glycoprotein 3; sperm receptor; female fertilization protein; positive selection; Cricetidae; Murinae

BORIS DOI:

10.48350/164201

URI:

https://boris.unibe.ch/id/eprint/164201

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