DNA Methyltransferase 3 (MET3) is regulated by Polycomb group complex during Arabidopsis endosperm development

Tirot, Louis; Bonnet, Diane M. V.; Jullien, Pauline E. (2022). DNA Methyltransferase 3 (MET3) is regulated by Polycomb group complex during Arabidopsis endosperm development. Plant reproduction, 35(2), pp. 141-151. Springer Nature 10.1007/s00497-021-00436-x

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Complex epigenetic changes occur during plant reproduction. These regulations ensure the proper transmission of epigenetic information as well as allowing for zygotic totipotency. In Arabidopsis, the main DNA methyltransferase is called MET1 and is responsible for methylating cytosine in the CG context. The Arabidopsis genome encodes for three additional reproduction-specific homologs of MET1, namely MET2a, MET2b and MET3. In this paper, we show that the DNA methyltransferase MET3 is expressed in the seed endosperm and its expression is later restricted to the chalazal endosperm. MET3 is biallelically expressed in the endosperm but displays a paternal expression bias. We found that MET3 expression is regulated by the Polycomb complex proteins FIE and MSI1. Seed development is not impaired in met3 mutant, and we could not observe significant transcriptional changes in met3 mutant. MET3 might regulates gene expression in a Polycomb mutant background suggesting a further complexification of the interplay between H3K27me3 and DNA methylation in the seed endosperm.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Epigenetics
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

Tirot, Louis Jean François, Bonnet, Diane Marie Valérie J., Jullien, Pauline Emilie

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2194-7953

Publisher:

Springer Nature

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

24 Feb 2022 16:34

Last Modified:

05 Dec 2022 16:07

Publisher DOI:

10.1007/s00497-021-00436-x

PubMed ID:

35088155

Uncontrolled Keywords:

DNA methylation, Arabidopsis, Endosperm, Polycomb, MET3

BORIS DOI:

10.48350/165231

URI:

https://boris.unibe.ch/id/eprint/165231

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