Replication and single-cycle delivery of SARS-CoV-2 replicons.

Ricardo-Lax, Inna; Luna, Joseph M; Tran, Thi Nhu Thao; Le Pen, Jérémie; Yu, Yingpu; Hoffmann, H-Heinrich; Schneider, William M; Razooky, Brandon S; Fernandez-Martinez, Javier; Schmidt, Fabian; Weisblum, Yiska; Trüeb, Bettina Salome; Berenguer Veiga, Inês; Schmied, Kimberly; Ebert, Nadine; Michailidis, Eleftherios; Peace, Avery; Sánchez-Rivera, Francisco J; Lowe, Scott W; Rout, Michael P; ... (2021). Replication and single-cycle delivery of SARS-CoV-2 replicons. Science, 374(6571), pp. 1099-1106. American Association for the Advancement of Science 10.1126/science.abj8430

[img]
Preview
Text
Thiel_Replication_and_single-cycle_delivery_science.abj8430.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Tran, Thi Nhu Thao; Trüeb, Bettina Salome; Berenguer Veiga, Inês; Ebert, Nadine and Thiel, Volker Earl

Subjects:

500 Science
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0036-8075

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Katharina Gerber-Paizs

Date Deposited:

24 Feb 2022 15:32

Last Modified:

03 Mar 2022 15:47

Publisher DOI:

10.1126/science.abj8430

PubMed ID:

34648371

BORIS DOI:

10.48350/165632

URI:

https://boris.unibe.ch/id/eprint/165632

Actions (login required)

Edit item Edit item
Provide Feedback