Aspiration toxicology of hydrocarbons and lamp oils studied by in vitro technology

Schneider, Sarah; Schürch, David; Geiser, Marianne (2013). Aspiration toxicology of hydrocarbons and lamp oils studied by in vitro technology. Toxicology in vitro, 27(3), pp. 1089-101. Oxford: Elsevier 10.1016/j.tiv.2013.01.015

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Medical literature regularly reports on accidental poisoning in children after aspiration of combustibles such as lamp oils which usually contain hydrocarbons or rape methyl esters (RMEs). We aimed to analyze the toxic potential of alkanes and different combustible classes in vitro with regard to biologic responses and mechanisms mediating toxicity. Two different in vitro models were used, i.e. (i) a captive bubble surfactometer (CBS) to assess direct influence of combustibles on biophysical properties of surfactant film and (ii) cell cultures (BEAS-2B and R3/1 cells, primary macrophages, re-differentiated epithelia) closely mimicking the inner lung surface. Biological endpoints included cell viability, cytotoxicity and inflammatory mediator release. CBS measurements demonstrate that combustibles affect film dynamics, i.e. the surface tension/area characteristics during compression and expansion, in a dose and molecular chain length dependent manner. Cell culture results confirm the dose dependent toxicity. Generally, cytotoxicity and cytokine release are higher in short-chained alkanes and hydrocarbon-based combustibles than in long-chained substances, e.g. highest inducible cytotoxicity in BEAS-2B was for hexane 84.6%, decane 74% and hexadecane 30.8%. Effects of RME-based combustibles differed between the cell models. Our results confirm data from animal experiments and give new insights into the mechanisms underlying the adverse health effects observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Schneider, Sarah; Schürch, David and Geiser, Marianne

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0887-2333

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:41

Last Modified:

18 Jan 2017 10:29

Publisher DOI:

10.1016/j.tiv.2013.01.015

PubMed ID:

23376439

Web of Science ID:

000317446500013

BORIS DOI:

10.7892/boris.16570

URI:

https://boris.unibe.ch/id/eprint/16570 (FactScience: 224238)

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