Shao, Feng; Panahipour, Layla; Sordi, Mariane Beatriz; Tang, Fangrui; Liu, Ronghua; Gruber, Reinhard (2022). Heartwood of Dalbergia cochinchinensis: 4,7,2'-Trihydroxy-4'-methoxyisoflavanol and 6,4'-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro. Molecules, 27(4) Molecular Diversity Preservation International MDPI 10.3390/molecules27041321
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Dalbergia cochinchinensis has been widely used in traditional medicine because of its flavonoids; however, the impact of the flavonoids to modulate the inflammatory response to oral cells remains to be described. For this aim, we isolated 4,7,2'-trihydroxy-4'-methoxyisoflavanol (472T4MIF) and 6,4'-dihydroxy-7-methoxyflavane (64D7MF) from the heartwood of D. cochinchinensis and confirmed the chemical structure by nuclear magnetic resonance. We show here that both flavonoids are inhibitors of an inflammatory response of murine RAW 264.7 inflammatory macrophages stimulated by LPS. This is indicated by interleukin (IL)1, IL6, and chemokine CCL2 production besides the phosphorylation of p65. Consistently, in primary murine macrophages, both flavonoids decreased the inflammatory response by lowering LPS-induced IL1 and IL6 expression. To introduce oral cells, we have used human gingival fibroblasts and provoked the inflammatory response by exposing them to IL1β and TNFα. Under these conditions, 472T4MIF, but not 64D7MF, reduced the expression of chemokines CXCL1 and CXCL2. Taken together, we identified two flavonoids that can reduce the expression of cytokines and chemokines in macrophages and fibroblastic cells.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1420-3049 |
Publisher: |
Molecular Diversity Preservation International MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
01 Mar 2022 14:42 |
Last Modified: |
05 Dec 2022 16:11 |
Publisher DOI: |
10.3390/molecules27041321 |
PubMed ID: |
35209110 |
Uncontrolled Keywords: |
Dalbergia cochinchinensis fibroblasts flavonoids macrophages |
BORIS DOI: |
10.48350/166096 |
URI: |
https://boris.unibe.ch/id/eprint/166096 |
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